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A Simple and Efficient Photoaffinity Method for Proteomics of GTP‐Binding Proteins
Author(s) -
Kaneda Masaki,
Masuda Souta,
Tomohiro Takenori,
Hatanaka Yasumaru
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600527
Subject(s) - diazirine , gtp' , photoaffinity labeling , chemistry , computational biology , simple (philosophy) , combinatorial chemistry , proteomics , biochemistry , binding site , biology , enzyme , philosophy , epistemology , gene
Multipurpose chemical . The diazirine‐carrying GTP analogue shown in the scheme can be used for various purposes, such as, carbene‐mediated cross‐linking, tagging, and affinity isolation. Using this GTP analogue, we have developed a simple and efficient photoaffinity method that can be employed in proteomic analysis of GTP‐binding proteins.