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The In Vitro Characterization of the Erythronolide Mycarosyltransferase EryBV and Its Utility in Macrolide Diversification
Author(s) -
Zhang Changsheng,
Fu Qiang,
Albermann Christoph,
Li Lingjun,
Thorson Jon S.
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600509
Subject(s) - diversification (marketing strategy) , in vitro , glycosyltransferase , erythromycin , chemistry , stereochemistry , biochemistry , combinatorial chemistry , computational biology , biology , antibiotics , enzyme , business , marketing
By capitalizing upon the reversibility of glycosyltransferase‐catalyzed reactions, EryBV was characterized in vitro as the erythromycin mycarosyltransferase and the EryBV substrate specificity was investigated. Two‐GT (EryBV/AveBI) aglycon‐exchange reactions also led to the production of novel hybrid “erythromycin–avermectin” macrolides.