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Seeing Is Believing: Peptide‐Based Fluorescent Sensors of Protein Tyrosine Kinase Activity
Author(s) -
Lawrence David S.,
Wang Qunzhao
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600473
Subject(s) - tyrosine , tyrosine kinase , sh2 domain , biochemistry , protein tyrosine phosphatase , proto oncogene tyrosine protein kinase src , receptor tyrosine kinase , sh3 domain , effector , kinase , microbiology and biotechnology , chemistry , biology , signal transduction
Protein tyrosine kinases are key biochemical effectors of the signaling pathways that drive both normal and aberrant cell behavior. The ability to visualize the activity of tyrosine kinases in both a continuous and sensitive fashion will have a dramatic impact on the identification and characterization of inhibitors, the elucidation of the biochemical role of protein tyrosine kinases in various biological processes, and the imaging of kinase action in cells, tissues, and whole organisms. Several chemical strategies have recently been described that translate the formation of a phosphorylated tyrosine residue into a fluorescent readout. The challenges associated with the design of protein tyrosine kinase sensors, as well as the scope and limitations of the currently available sensors, are described.