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Highly Efficient Chemoenzymatic Synthesis of Novel Branched Thiooligosaccharides by Substrate Direction with Glucansucrases
Author(s) -
Hellmuth Hendrik,
Hillringhaus Lars,
Höbbel Sven,
Kralj Slavko,
Dijkhuizen Lubbert,
Seibel Jürgen
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600444
Subject(s) - chemoselectivity , substrate (aquarium) , glycoconjugate , chemistry , combinatorial chemistry , biocatalysis , substrate specificity , stereochemistry , catalysis , enzyme , biochemistry , biology , reaction mechanism , ecology
Various complex glycoconjugates containing thioglycosidic linkages have been constructed in high yields by using a two‐step chemoenzymatic approach. The chemoselectivity of the used glucansucrases can be guided from α‐1,6 to α‐1,2, α‐1,3 or α‐1,4 linked glucose by the right choice of the acceptor substrate (substrate engineering) providing a powerful tool for diverse glycosynthesis.