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Functional Immobilization of the Small GTPase Rab6A on DNA–Gold Nanoparticles by Using a Site‐Specifically Attached Poly(ethylene glycol) Linker and Thiol Place‐Exchange Reaction
Author(s) -
Becker Christian F. W.,
Marsac Yoann,
Hazarika Pompi,
Moser Jens,
Goody Roger S.,
Niemeyer Christof M.
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600422
Subject(s) - linker , ethylene glycol , colloidal gold , peg ratio , thiol , oligonucleotide , nanoparticle , chemistry , combinatorial chemistry , ligand (biochemistry) , dna , nanotechnology , organic chemistry , biochemistry , materials science , computer science , receptor , finance , economics , operating system
PEG'ed to gold . An approach for the functional immobilization of proteins via a thiol‐modified PEG linker on gold nanoparticles is described. A place‐exchange reaction of thiolated oligonucleotides with PEG‐modified proteins leads to stable protein–nanoparticle conjugates.