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A Molecular Probe for the Detection of Homopurine Sequences
Author(s) -
Trkulja Ivan,
Biner Sarah M.,
Langenegger Simon M.,
Häner Robert
Publication year - 2007
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600378
Subject(s) - oligonucleotide , pyrene , chemistry , excimer , nucleotide , sequence (biology) , molecular probe , computational biology , combinatorial chemistry , biophysics , biochemistry , fluorescence , gene , biology , physics , organic chemistry , quantum mechanics
The highly selective detection of homopurine target strands with a triplex‐forming molecular probe is described. The binding of a clamp‐type oligonucleotide containing two terminally attached pyrene molecules to the target sequence is easily monitored through excimer formation. The oligonucleotide probe allows the efficient discrimination of single nucleotide mismatches because of the high mismatch sensitivity of the triplex formation.