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Intracellular Imaging of HCV RNA and Cellular Lipids by Using Simultaneous Two‐Photon Fluorescence and Coherent Anti‐Stokes Raman Scattering Microscopies
Author(s) -
Nan Xiaolin,
Tonary Angela M.,
Stolow Albert,
Xie X. Sunney,
Pezacki John Paul
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600330
Subject(s) - rna , raman scattering , raman spectroscopy , fluorescence , intracellular , microscopy , subcellular localization , hepatitis c virus , fluorescence microscope , biophysics , photon , chemistry , microbiology and biotechnology , biology , biochemistry , virology , cytoplasm , physics , gene , virus , optics
Hepatitis C virus (HCV) infection is associated with changes in host‐cell lipid metabolism. Here we describe a new approach for detecting HCV RNA using two‐photon fluorescence (TPF), and HCV‐associated changes in cellular lipids using coherent anti‐Stokes Raman scattering (CARS) microscopy. By combining the two types of microscopy with a common laser source, we visualized both phenomena simultaneously and profiled cellular lipids and subcellular localization of RNA in real time.