Premium
Site‐Specific Immobilization of Genetically Engineered Variants of Candida antarctica Lipase B
Author(s) -
Blank Kerstin,
Morfill Julia,
Gaub Hermann E.
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600198
Subject(s) - candida antarctica , lipase , chemistry , computational biology , cysteine , covalent bond , directed evolution , mutant , biochemistry , biology , enzyme , gene , organic chemistry
Keep still! A general strategy has been developed for the site‐specific and covalent immobilization of biomolecules. The applicability of this protocol was demonstrated by using the industrially important lipase CalB. Two mutants that possess a free cysteine (Cys293 or C‐terminal) were prepared and immobilized in an active conformation at the desired position.