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Single Amino Acid Substitutions Alter the Efficiency of Docking in Modular Polyketide Biosynthesis
Author(s) -
Weissman Kira J.
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600185
Subject(s) - polyketide , docking (animal) , biosynthesis , modular design , amino acid , stereochemistry , mutagenesis , computational biology , biology , chemistry , biochemistry , enzyme , computer science , gene , mutation , programming language , medicine , nursing
It only takes one . Polyketide biosynthesis on modular polyketide synthases (PKSs) depends on the specific interaction between multiple protein subunits. These recognition events are mediated in part by docking domains at the termini of the multienzymes. Site‐directed mutagenesis within a model PKS system shows that even single amino acid substitutions within the docking domains can significantly alter the efficiency of docking.