Leucyl/Phenylalanyl‐tRNA‐Protein Transferase‐Mediated Chemoenzymatic Coupling of N‐Terminal Arg/Lys Units in Post‐translationally Processed Proteins with Non‐natural Amino Acids | Zendy

Taki Masumi | Zendy; Kuno Atsushi | Zendy; Matoba Shinsuke | Zendy; Kobayashi Yuki | Zendy; Futami Junichiro | Zendy; Murakami Hiroshi | Zendy; Suga Hiroaki | Zendy; Taira Kazunari | Zendy; Hasegawa Tsunemi | Zendy; Sisido Masahiko | Zendy

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Leucyl/Phenylalanyl‐tRNA‐Protein Transferase‐Mediated Chemoenzymatic Coupling of N‐Terminal Arg/Lys Units in Post‐translationally Processed Proteins with Non‐natural Amino Acids

Author(s) -

Taki Masumi,

Kuno Atsushi,

Matoba Shinsuke,

Kobayashi Yuki,

Futami Junichiro,

Murakami Hiroshi,

Suga Hiroaki,

Taira Kazunari,

Hasegawa Tsunemi,

Sisido Masahiko

Publication year - 2006

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200600181

Subject(s) - transfer rna , amino acid , transferase , biochemistry , chemistry , biology , stereochemistry , enzyme , rna , gene

The protein no longer terminates here . Non‐natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F‐tRNA‐protein transferase to the N termini of proteins possessing N‐terminal Lys or Arg components. By this new technique, 1‐ and 2‐naphthylalanine or 3‐nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.

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