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Leucyl/Phenylalanyl‐tRNA‐Protein Transferase‐Mediated Chemoenzymatic Coupling of N‐Terminal Arg/Lys Units in Post‐translationally Processed Proteins with Non‐natural Amino Acids
Author(s) -
Taki Masumi,
Kuno Atsushi,
Matoba Shinsuke,
Kobayashi Yuki,
Futami Junichiro,
Murakami Hiroshi,
Suga Hiroaki,
Taira Kazunari,
Hasegawa Tsunemi,
Sisido Masahiko
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600181
Subject(s) - transfer rna , amino acid , transferase , biochemistry , chemistry , biology , stereochemistry , enzyme , rna , gene
The protein no longer terminates here . Non‐natural amino acids were attached onto a tRNA and were then transferred with the aid of L/F‐tRNA‐protein transferase to the N termini of proteins possessing N‐terminal Lys or Arg components. By this new technique, 1‐ and 2‐naphthylalanine or 3‐nitrotyrosine were coupled to the N termini of several proteins. A novel and convenient method for adding single Lys units at the N termini of various proteins was also developed.