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Synthesis and Evaluation of an Intercalator–Polyamide Hairpin Designed to Target the Inverted CCAAT Box 2 in the Topoisomerase IIα Promoter
Author(s) -
Flores Lloyd V.,
Staples Andrew M.,
Mackay Hilary,
Howard Cameron M.,
Uthe Peter B.,
Sexton Jim S.,
Buchmueller Karen L.,
Wilson W. David,
O'Hare Caroline,
Kluza Jerome,
Hochhauser Daniel,
Hartley John A.,
Lee Moses
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600155
Subject(s) - moiety , stereochemistry , binding site , binding constant , chemistry , dna , footprinting , intercalation (chemistry) , dissociation constant , dna footprinting , caat box , crystallography , promoter , transcription factor , dna binding protein , biochemistry , receptor , gene , inorganic chemistry , gene expression , base sequence
Abstract The synthesis and DNA‐binding properties of a novel naphthalimide–polyamide hairpin ( 3 ) designed to target the inverted CCAAT box 2 (ICB2) site on the topoisomerase IIα (topoIIα) promoter are described. The polyamide component of 3 was derived from the minor‐groove binder, 2 , and tailored to bind to the 5′‐TTGGT sequence found in and flanking ICB2. The propensity of mitonafide 4 to intercalate between G–C base pairs was exploited by the incorporation of a naphthalimide moiety at the N terminus of 2 . Hybrid 3 targeted 5′‐CG ATTGG T and covered eight contiguous base pairs, which included the underlined ICB2 site. DNase I footprinting analysis with the topoIIα promoter sequence demonstrated that 3 bound selectively to the ICB2 and ICB3 sites. Thermal‐denaturation studies confirmed these results, and the highest degree of stabilization was found for ICB2 and ‐3 in preference to ICB1 (4.1, 4.6, and 0.6 °C, respectively). CD studies confirmed minor‐groove binding and suggested a 1:1 binding stoichiometry. Emission‐titration experiments established intercalative binding. Surface plasmon resonance results showed strong binding to ICB2 (2.5×10 7 M −1 ) with no observable binding to ICB1. Furthermore, the binding constant of 3 to ICB2 was larger than that of the parent polyamide 2 . The increased binding affinity was primarily due to a reduction in the dissociation‐rate constant of the polyamide–DNA complex, which can be attributed to the N‐terminal naphthalimide moiety. In addition, the binding site of 3 was larger than that of 2 , which innately improved sequence selectivity. We conclude that the polyamide–naphthalimide 3 selectively binds to the ICB2 site by simultaneous intercalation and minor‐groove binding, and warrants further investigation as a model compound for the regulation of topoIIα gene expression.