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Detection of mRNA in Mammalian Cells with a Split Ribozyme Reporter
Author(s) -
Hasegawa Sumitaka,
Gowrishankar Gayatri,
Rao Jianghong
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600061
Subject(s) - ribozyme , messenger rna , rna , reporter gene , tetrahymena , intron , mammalian cpeb3 ribozyme , microbiology and biotechnology , biology , mutant , gene , gene expression , genetics
A Tetrahymena group I intron ribozyme reporter was split into two halves, and, in the presence of the target mRNA, the split reporter assembled into an active complex that trans ‐spliced and produced a reporter enzyme. We show that this split reporter can sense tumor‐suppressor p53 mutant mRNA in mammalian cells. This system may open up new avenues for detecting and imaging RNA molecules in vivo.