Premium
Stepwise Solid‐Phase Synthesis and Spontaneous Homodimerization of the Helix‐Loop‐Helix Protein Id3
Author(s) -
Svobodová Jaroslava,
Cabrele Chiara
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200600059
Subject(s) - solid phase synthesis , bridging (networking) , chemistry , residue (chemistry) , disulfide bond , helix (gastropod) , combinatorial chemistry , stereochemistry , computational biology , biochemistry , biology , computer science , peptide , snail , ecology , computer network
Chemical brothers . Id3, a 119‐residue‐long protein inhibitor of DNA binding and cell differentiation, was prepared by solid‐phase methodology (SPPS) and Fmoc chemistry (see figure). The synthetic product was prone to self‐association, and homodimers that were stabilized by disulfide bridging could be detected by SDS‐PAGE.