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C‐Terminal Fluorescence Labeling of Proteins for Interaction Studies on the Single‐Molecule Level
Author(s) -
Becker Christian F. W.,
Seidel Ralf,
Jahnz Michael,
Bacia Kirsten,
Niederhausen Thomas,
Alexandrov Kirill,
Schwille Petra,
Goody Roger S.,
Engelhard Martin
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500535
Subject(s) - förster resonance energy transfer , fluorescence , chemistry , molecule , biophysics , single molecule experiment , protein–protein interaction , bimolecular fluorescence complementation , energy transfer , biochemistry , biology , gene , organic chemistry , chemical physics , physics , quantum mechanics
Homogeneously labeled protein samples are generated by expressed protein ligation for studying protein interactions. Miniscule amounts of samples are used in single‐molecule‐sensitive measurements and fluorescence resonance energy transfer experiments.