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Template‐Directed DNA Photoligation in Rapid and Selective Detection of RNA Point Mutations
Author(s) -
Yoshimura Yoshinaga,
Noguchi Yuuki,
Sato Hideaki,
Fujimoto Kenzo
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500534
Subject(s) - rna , dna , computational biology , point mutation , microbiology and biotechnology , chemistry , biology , gene , genetics , mutation
Spotlight on RNA sequences . Efficient photoligation, without any by‐product formation, was observed when a carboxyvinyl‐2′‐deoxyuridine‐containing oligodeoxynucleotide was photoirradiated with one containing a pyrimidine base at the 3′ end in the presence of template RNA. Furthermore, RNA mismatches can be successfully distinguished by using a DNA‐chip assay based on this photoligation. Therefore, this system could be widely used for the detection of RNA sequences.