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High‐Throughput‐Compatible Assay for glmS Riboswitch Metabolite Dependence
Author(s) -
Mayer Günter,
Famulok Michael
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500490
Subject(s) - ribozyme , riboswitch , metabolite , computational biology , chemistry , cleavage (geology) , rna , biology , biochemistry , gene , non coding rna , paleontology , fracture (geology)
A fluorescent varient of the glmS ribozyme was used to develop a fluorescence polarization‐dependent screeening and metabolite‐detection assay. The metabolite‐induced in‐cis cleavage of the naturally occurring ribozyme could be shown to be stable and allows efficient monitoring of the ribozyme‐catalyzed reaction even in bacterial extracts. The standard deviations and z ′ values of the assay format indicate the high‐throughput screening compatibility of the applied system for the identification of compounds that act on RNA structures.