Premium
Utilization of L ‐α‐Nucleobase Amino Acids (NBAs) as Protein Engineering Tools: Construction of NBA‐Modified HIV‐1 Protease Analogues and Enhancement of Dimerization Induced by Nucleobase Interaction
Author(s) -
Takahashi Tsuyoshi,
Yana Dewi,
Mihara Hisakazu
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500422
Subject(s) - nucleobase , chemistry , protease , protein–protein interaction , protein engineering , amino acid , human immunodeficiency virus (hiv) , combinatorial chemistry , biochemistry , dna , biology , enzyme , virology
Takes one to know one . We have applied homo base‐pairing interactions, such as C–C + and G–G, to a specific enhancer in the dimerization domain of the HIV‐1 protease subunit by using nucleobase amino acids (NBAs). Incorporation of NBAs into the dimerization domain of the protease (see figure), which resulted in C–C + and G–G homo base pairs, enhanced its dimerization ability and catalytic activity.