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DNA‐Templated Synthesis in Organic Solvents
Author(s) -
Rozenman Mary M.,
Liu David R.
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500413
Subject(s) - dna , aqueous solution , chemistry , aldol condensation , condensation , organic solvent , condensation reaction , organic synthesis , combinatorial chemistry , organic chemistry , catalysis , chemical engineering , biochemistry , engineering , physics , thermodynamics
When DNA duplexes are preformed in water , then DNA‐templated synthesis (DTS) can take place efficiently and sequence‐specifically in organic solvents with low or minimal water content. This simple method enables reactions that are inaccessible in water, such as pyrrolidine‐catalyzed aldol condensation, to be performed. The conditions needed to perform DTS in organic solvents also support DNA‐templated reactions that are known to take place in aqueous solution.