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Polysaccharide Microarrays for Polysaccharide–Platelet‐Derived‐Growth‐Factor Interaction Studies
Author(s) -
Carion Olivier,
Lefebvre Julien,
Dubreucq Guy,
DahriCorreia Latifa,
Correia José,
Melnyk Oleg
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500387
Subject(s) - polysaccharide , chemistry , growth factor , dna microarray , microarray , microarray analysis techniques , biochemistry , platelet factor 4 , gene expression , gene , receptor , heparin
Polysaccharide microarrays have great potential for the high‐throughput analysis of polysaccharide–protein interactions. Here we demonstrate that a polysaccharide microarray prepared by printing a library of dextran polymers derivatized by methylcarboxylate, benzylamide, and sulfate groups (DMCBSu compounds) on to glass slides permitted the rapid identification of a set of compounds able to interact with the platelet‐derived growth factor BB (PDGF‐BB) isoform, a growth factor involved in wound healing. Microarray interaction results were compared to the capacity of DMCBSu compounds to potentiate the in vitro PDGF‐BB‐induced proliferation of human dermal fibroblasts.