Premium
Miniaturization of Microwave‐Assisted Carbohydrate Functionalization to Create Oligosaccharide Microarrays
Author(s) -
Brun Matthias A.,
Disney Matthew D.,
Seeberger Peter H.
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500361
Subject(s) - glycoconjugate , dna microarray , oligosaccharide , miniaturization , chemistry , carbohydrate , microwave , computational biology , computer science , nanotechnology , biochemistry , biology , materials science , gene expression , gene , telecommunications
A microwave‐assisted Kochetkov reaction (a) has been used on different reducing carbohydrates to obtain glycosylamines and then N ‐iminoglycosylamidines without purification. These glycoconjugates were then used to construct microarrays to test known carbohydrate–protein interactions. This method streamlines efforts required to functionalize carbohydrates for construction of arrays.