Expanding the Genetic Code in a Mammalian Cell Line by the Introduction of Four‐Base Codon/Anticodon Pairs | Zendy

Taki Masumi | Zendy; Matsushita Jiro | Zendy; Sisido Masahiko | Zendy

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Expanding the Genetic Code in a Mammalian Cell Line by the Introduction of Four‐Base Codon/Anticodon Pairs

Author(s) -

Taki Masumi,

Matsushita Jiro,

Sisido Masahiko

Publication year - 2006

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200500360

Subject(s) - genetic code , transfer rna , stop codon , base pair , codon usage bias , gene , base (topology) , start codon , genetics , biology , code (set theory) , line (geometry) , computational biology , computer science , rna , base sequence , genome , programming language , mathematics , mathematical analysis , geometry , set (abstract data type)

Switching code at Tyr66. We report the expansion of the genetic code in a mammalian cell line by using various four‐base codons. These were derived either from an amber stop codon or rare codons from mammalian cells. Among the four‐base codon/anticodon pairs examined, the UAGG/ccua pair was the most efficiently decoded and expressed the highest amount of enhanced green fluorescent protein (EGFP; see scheme).

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