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Identification of New Acceptor Specificities of Glycosyltransferase R with the Aid of Substrate Microarrays
Author(s) -
Seibel Jürgen,
Hellmuth Hendrik,
Hofer Bernd,
Kicinska AnnaMaria,
Schmalbruch Bodo
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500350
Subject(s) - glycosyltransferase , biochemistry , glycosylation , chemistry , amino acid , carbohydrate synthesis , glycobiology , dna microarray , enzyme , biology , glycoprotein , gene , glycan , gene expression
Finding opportunities to construct sugar motifs and to transfer them to targets of biological relevance and rapid identification of glycosylation events are important goals for glycobiology and a field of increasing interest. Here we have applied an enzyme microarray screening system for the identification of new acceptor specificities of the glycosyltransferase R (GTFR) from Streptococcus oralis (E.C. 2.4.1.5), which was able to effect the synthesis of sugar motifs in short times and with low amounts of substrate. These observations resulted in the development of a convenient α‐glycosylation by the non‐Leloir glycosyltransferase GTFR, with sucrose as substrate and with different alcohols and amino acid derivatives as acceptors, for the synthesis of glycoethers and glycosylated amino acids not observed with the use of familiar GTFs with high sequence homology.