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Activity‐Based Fingerprinting of Proteases
Author(s) -
Srinivasan Rajavel,
Huang Xuan,
Ng Su Ling,
Yao Shao Q.
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500340
Subject(s) - proteases , computational biology , enzyme , chemistry , proteomics , substrate specificity , biochemistry , biology , gene
Not inhibited . We have successfully synthesized and tested a panel of activity‐based small‐molecule probes that target different classes of proteases on the basis of their enzymatic activities and substrate specificities rather than their inhibition. We have demonstrated that these probes are useful for generating unique substrate fingerprint profiles of proteases in gel‐based proteomic experiments. Preliminary results indicate that they might be equally amenable for microarray‐based enzyme profiling experiments.