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Chromogenic and Fluorogenic Assays for the Lactonase Activity of Serum Paraoxonases
Author(s) -
Khersonsky Olga,
Tawfik Dan S.
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500334
Subject(s) - chromogenic , chemistry , pon1 , enzyme , biochemistry , paraoxonase , chromatography , microbiology and biotechnology , biology , genotype , gene
5‐Thioalkyl butyrolactones (1) are shown to be useful chromogenic/fluorogenic probes for determining lactonase activities, in particular of serum paraoxonase (PON1). These novel probes are suitable for measuring lactonase activity in complex biological samples, including sera, intact cells and cell lysates.