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Selective Staudinger Modification of Proteins Containing p ‐Azidophenylalanine
Author(s) -
Tsao MengLin,
Tian Feng,
Schultz Peter G.
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500314
Subject(s) - azide , posttranslational modification , mutant , chemistry , peptide , computational biology , combinatorial chemistry , protein engineering , amino acid , biochemistry , biology , gene , organic chemistry , enzyme
The unnatural amino acid p ‐azidophenylalanine was genetically introduced into a phage‐displayed peptide and mutant Z‐domain protein. These azide‐containing proteins were selectively and efficiently modified by using fluorescein‐derived phosphines and the Staudinger ligation reaction (see picture). This work provides a powerful new tool for the selective modification of proteins with a variety of synthetic agents.