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Tetrabutylammonium Fluoride‐Mediated Rapid Alkylation Reaction in Microtiter Plates for the Discovery of Enzyme Inhibitors in Situ
Author(s) -
Wu ChungYi,
Brik Ashraf,
Wang ShengKai,
Chen YuHsien,
Wong ChiHuey
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500295
Subject(s) - enzyme , in situ , chemistry , microtiter plate , alkylation , protease , tetrabutylammonium fluoride , fluoride , combinatorial chemistry , chromatography , biochemistry , organic chemistry , catalysis , inorganic chemistry
No isolation, no purification . An efficient and simple method based on tetrabutylammonium fluoride‐mediated alkylation without protecting groups in microtiter plates, followed by screening in situ has been developed to identify new enzyme inhibitors, as demonstrated in the discovery of the shown potent inhibitors of cathepsin B and HIV protease.