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Nonenzymatic Ligation of Short‐Chained 2′–5′‐ or 3′–5′‐Linked Oligoribonucleotides on 2′–5′‐ or 3′–5′‐Linked Complementary Templates
Author(s) -
Sawai Hiroaki,
Wada Makoto,
Kouda Tsukasa,
Nakamura Ozaki Akiko
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500291
Subject(s) - tetramer , histone octamer , chemistry , template , stereochemistry , substrate (aquarium) , ligation , crystallography , dna , biochemistry , enzyme , nanotechnology , biology , materials science , microbiology and biotechnology , ecology , nucleosome , histone
5′‐pACUG tetraribonucleotides containing 2′–5′ or 3′–5′ linkages self‐condensed on 2′–5′‐ or 3′–5′‐linked complementary decaribonucleotide (5′‐CAGUCAGUCA) templates. CD and UV melting studies showed that helix formation took place in all four possible combinations of linkage isomers of the substrate tetramer and the template decamer under the ligation conditions. The hybridization ability followed the order: [3′–5′ tetramer with 3′–5′ decamer]>[2′–5′ tetramer with 3′–5′ decamer]>[2′–5′ tetramer with 2′–5′ decamer]≥[3′–5′ tetramer with 2′–5′ decamer]. Each tetramer condensed on the complementary decaribonucletide template to form the corresponding octamer, but the ligation efficiency varied considerably, depending on the types of linkage in the tetramer substrate and the template decamer. The yields of the octamers obtained by the template‐directed ligation followed the order: [2′–5′ substrate: 2′–5′ template]>[3′–5′:3′–5′]>[2′–5′:3′–5′]>[3′–5′:2′–5′]. The results demonstrate that a homo‐linkage system is preferable for the template‐directed synthesis of RNA. The resulting linkage of the octamer formed from the 2′–5′‐linked substrate and the 2′–5′‐linked template is mainly 2′–5′.