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Carbon Nanotube Delivery of the GFP Gene into Mammalian Cells
Author(s) -
Gao Lizeng,
Nie Leng,
Wang Taihong,
Qin Yujun,
Guo Zhixin,
Yang Dongling,
Yan Xiyun
Publication year - 2006
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500227
Subject(s) - green fluorescent protein , gene delivery , carbon nanotube , cytotoxicity , dna , nanotechnology , gene , biophysics , chemistry , fluorescence , microbiology and biotechnology , genetic enhancement , materials science , biochemistry , biology , in vitro , physics , quantum mechanics
Special delivery. Amino‐functionalized multiwalled carbon nanotubes (NH 2 ‐MWCNTs) were found to interact with negatively charged DNA and the cell membrane (see figure). Through this interaction, NH 2 ‐MWCNTs successfully delivered the GFP (green fluorescent protein) gene into mammalian cells without any cytotoxicity. The results show that NH 2 ‐MWCNT is a novel gene carrier and has potential application in gene delivery.