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Site‐Specific Fluorescent and Affinity Labelling of RNA by Using a Small Engineered Twin Ribozyme
Author(s) -
Vauléon Stéphanie,
Ivanov Sergei A.,
Gwiazda Slawomir,
Müller Sabine
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500215
Subject(s) - ribozyme , oligonucleotide , rna , computational biology , ligase ribozyme , chemistry , labelling , fluorescence , combinatorial chemistry , biology , computer science , biochemistry , gene , physics , quantum mechanics
Site‐determined functionalization of large RNA molecules can be achieved by a strategy involving twin ribozymes and small synthetic oligonucleotides. The twin ribozyme mediates the exchange of a predefined patch of RNA sequence against a modified oligonucleotide to result in specific functionalization of the large RNA.