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Fluorescent Detection of Apoptotic Cells by Using Zinc Coordination Complexes with a Selective Affinity for Membrane Surfaces Enriched with Phosphatidylserine
Author(s) -
Hanshaw Roger G.,
Lakshmi C.,
Lambert Timothy N.,
Johnson James R.,
Smith Bradley D.
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500149
Subject(s) - phosphatidylserine , membrane , fluorescence , chemistry , photobleaching , hela , jurkat cells , biophysics , fluorescence recovery after photobleaching , organelle , apoptosis , phospholipid , cell , biochemistry , biology , physics , immune system , quantum mechanics , t cell , immunology
The appearance of phosphatidylserine on the membrane surface of apoptotic cells (Jurkat, CHO, HeLa) is monitored by using a family of bis(Zn 2+ ‐2,2′‐dipicolylamine) coordination compounds with appended fluorescein or biotin groups as reporter elements. The phosphatidylserine affinity group is also conjugated directly to a CdSe/CdS quantum dot to produce a probe suitable for prolonged observation without photobleaching. Apoptosis can be detected under a wide variety of conditions, including variations in temperature, incubation time, and binding media. Binding of each probe appears to be restricted to the cell membrane exterior, because no staining of organelles or internal membranes is observed.