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Probing Lectin and Sperm with Carbohydrate‐Modified Quantum Dots
Author(s) -
Robinson Anandakathir,
Fang JimMin,
Chou PiTai,
Liao KuangWen,
Chu ReaMin,
Lee ShyhJye
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500112
Subject(s) - wheat germ agglutinin , sperm , quantum dot , lectin , dynamic light scattering , biophysics , chemistry , confocal microscopy , flow cytometry , transmission electron microscopy , fluorescence microscope , microscopy , peanut agglutinin , fluorescence , agglutinin , nanotechnology , biochemistry , biology , microbiology and biotechnology , materials science , optics , physics , nanoparticle , genetics
We report the encapsulation of quantum dots with biologically important β‐ N ‐acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc‐encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose‐encapsulated QDs (QDMANs) with sperm revealed site‐specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body.