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Pseudoazurin–Nitrite Reductase Interactions
Author(s) -
Impagliazzo Antonietta,
Krippahl Ludwig,
Ubbink Marcellus
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500082
Subject(s) - chemistry , nitrite reductase , docking (animal) , nitrite , binding site , ligand (biochemistry) , hydrophobic effect , stereochemistry , biochemistry , organic chemistry , nitrate , receptor , medicine , nursing
The nitrite reductase‐binding site on pseudoazurin has been determined by using NMR chemical‐shift perturbations. It comprises residues in the hydrophobic patch surrounding the exposed copper ligand His81 as well as several positively charged residues. The binding site is similar for both redox states of pseudoazurin, despite differences in the binding mode. The results suggest that pseudoazurin binds in a well‐defined orientation. Docking simulations provide a putative structure of the complex with a binding site on nitrite reductase that has several hydrophobic and polar residues as well as a ridge of negatively charged side chains and a copper‐to‐copper distance of 14 Å.