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Luminescent Saccharide Biosensor by Using Lanthanide‐Bound Lectin Labeled with Fluorescein
Author(s) -
Koshi Yoichiro,
Nakata Eiji,
Hamachi Itaru
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500072
Subject(s) - fluorophore , luminescence , glycoconjugate , lanthanide , biosensor , chemistry , lectin , fluorescein , combinatorial chemistry , biochemistry , fluorescence , organic chemistry , materials science , optoelectronics , ion , physics , quantum mechanics
A new luminescent biosensor for complicated glycoconjugates was engineered on the basis of a lanthanide‐complexed sugar‐binding protein (lectin) modified with a fluorophore. By using luminescence resonance energy transfer (LRET), ratiometric luminescent sensing can be carried out and successfully applied to a luminescent assay for an enzymatic trimming of a glycoprotein (see scheme).