Luminescent Saccharide Biosensor by Using Lanthanide‐Bound Lectin Labeled with Fluorescein | Zendy

Koshi Yoichiro | Zendy; Nakata Eiji | Zendy; Hamachi Itaru | Zendy

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Luminescent Saccharide Biosensor by Using Lanthanide‐Bound Lectin Labeled with Fluorescein

Author(s) -

Koshi Yoichiro,

Nakata Eiji,

Hamachi Itaru

Publication year - 2005

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200500072

Subject(s) - fluorophore , luminescence , glycoconjugate , lanthanide , biosensor , chemistry , lectin , fluorescein , combinatorial chemistry , biochemistry , fluorescence , organic chemistry , materials science , optoelectronics , ion , physics , quantum mechanics

A new luminescent biosensor for complicated glycoconjugates was engineered on the basis of a lanthanide‐complexed sugar‐binding protein (lectin) modified with a fluorophore. By using luminescence resonance energy transfer (LRET), ratiometric luminescent sensing can be carried out and successfully applied to a luminescent assay for an enzymatic trimming of a glycoprotein (see scheme).

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