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Fluorescent Multiplex Analysis of Carrier Protein Post‐Translational Modification
Author(s) -
Mercer Andrew C.,
La Clair James J.,
Burkart Michael D.
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500051
Subject(s) - multiplex , fluorescence , posttranslational modification , computational biology , fusion protein , chemistry , biochemistry , fluorescent protein , green fluorescent protein , biology , bioinformatics , gene , enzyme , recombinant dna , physics , quantum mechanics
A multiplex assay to investigate the functional specificity between carrier proteins, phosphopantheinyl transferases, and coenzyme A (CoA) derivatives is shown. This system offers a multicolored tool for the biophysical study of biosynthetic pathways and orthogonally designed fusion proteins.