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Nanostructured Ordering of Fluorescent Markers and Single Proteins on Substrates
Author(s) -
Groll Juergen,
Albrecht Krystyna,
Gasteier Peter,
Riethmueller Silke,
Ziener Ulrich,
Moeller Martin
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500041
Subject(s) - fluorescence , monolayer , octadecyltrichlorosilane , fluorescence microscope , adsorption , chemistry , linker , nanotechnology , materials science , organic chemistry , physics , quantum mechanics , computer science , operating system
Highly ordered hexagonal nanopatterns of gold clusters on glass substrates were used as anchoring points for the specifc attachment of fluorescence dyes and proteins labeled with fluorescence dyes. Thiol‐ or disulfide‐containing linker molecules were used for the binding to the gold dots. In order to ensure specific binding on the gold dots only, the surface area in between the dots was protected against unspecific adsorption. For the attachment of polar low‐molecular‐weight fluorescence dyes, an octadecyltrichlorosilane self‐assembled monolayer was prepared on the surface in between the gold dots, whereas a layer prepared from star‐shaped poly(ethylene oxide‐ stat ‐propylene oxide) prepolymers was used to prevent unspecific adsorption of proteins between the gold dots. Fluorescence microscopy proved the specific binding of the dyes as well as of the proteins. Scanning force microscopy studies show that each gold dot is only capable of binding one protein at a time.