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Carbohydrate‐Encapsulated Gold Nanoparticles for Rapid Target‐Protein Identification and Binding‐Epitope Mapping
Author(s) -
Chen YuJu,
Chen ShuHua,
Chien YuhYih,
Chang YuWan,
Liao HsinKai,
Chang ChihYang,
Jan MiDan,
Wang KenTseng,
Lin ChunCheng
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500023
Subject(s) - epitope , carbohydrate , computational biology , lectin , chemistry , identification (biology) , biochemistry , colloidal gold , nanoparticle , nanotechnology , biology , antigen , genetics , botany , materials science
Carbohydrate–lectin recognition plays important roles in cell–cell communication, proliferation, and differentiation. We report here a new approach of using a carbohydrate‐encapsulated gold nanoparticle (shown in purple) as an affinity probe for the efficient separation and enrichment of target proteins, and then protein identification and epitope mapping by MALDI‐TOF MS.