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Fast Directed Evolution of Non‐Immunoglobulin Proteins by Somatic Hypermutation in Immune Cells
Author(s) -
Heinis Christian,
Johnsson Kai
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200500011
Subject(s) - somatic hypermutation , biology , somatic cell , phenotype , immune system , gene , antibody , mutation , genetics , immunoglobulin gene , b cell
New, improved phenotypes . Somatic hypermutation in immune cells can be used for the fast directed evolution of proteins other than immunoglobulins (e.g., autofluorescent proteins). The target gene is inserted into the genome of activated B lymphocytes where it is mutated. Cells expressing a desired phenotype are selected and subjected to further evolution cycles to accumulate beneficial mutations.