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Cover Picture: Two‐Dimensional Multiarray Formation of Hepatocyte Spheroids on a Microfabricated PEG‐Brush Surface (ChemBioChem 6/2004)
Author(s) -
Otsuka Hidenori,
Hirano Akihiro,
Nagasaki Yukio,
Okano Teruo,
Horiike Yasuhiro,
Kataoka Kazunori
Publication year - 2004
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200490024
Subject(s) - spheroid , peg ratio , microfabrication , adhesion , nanotechnology , chemistry , hepatocyte , biophysics , materials science , in vitro , biology , biochemistry , medicine , alternative medicine , organic chemistry , finance , pathology , fabrication , economics
The cover picture shows a simple and effective way to construct a 2D microarray of 10 000 hepatocyte heterospheroids, underlaid with endothelial cells, through a combination of surface PEG treatment and subsequent microfabrication of plasma etching. The micropatterned PEG‐treated region works to repel proteins and consequently inhibits cell adhesion, while the glass circular domains (Ø=100 μm), separated by PEG‐treated regions ( l =100 μm), provide cell adhesive regions. This spheroid array is very useful as a platform for tissue and cell‐based biosensors, which can be used as an alternative to animal experiments in a variety of situations. For more details, see the article by Kataoka et al. on p. 850 ff.