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High‐Throughput Evaluation of Antioxidant and Pro‐oxidant Activities of Polyphenols with Thymidine Protection Assays
Author(s) -
Meunier Stéphane,
Hanédanian Mikaël,
DesageEl Murr Marine,
Nowaczyk Stéphanie,
Le Gall Thierry,
Pin Serge,
Renault JeanPhilippe,
Boquet Didier,
Crémi Christophe,
Mioskowski Charles,
Taran Frédéric
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400421
Subject(s) - pro oxidant , antioxidant , chemistry , radiolysis , thymidine , oxidative stress , high throughput screening , polyphenol , combinatorial chemistry , biochemistry , oxidative phosphorylation , radical , dna
A recently reported high‐throughput screening strategy has been applied to the rapid selection of new water‐soluble antioxidants that display strong protective activities. Based on a competitive immunoassay, a triple‐screening procedure was used to evaluate the ability of different compounds to protect thymidine under different oxidative stresses. The pro‐oxidant effect of norbadione A in the presence of iron was observed, while some pulvinic acid derivatives proved strongly protective during γ radiolysis, UV irradiation, and Fenton‐like oxidation.