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The Oxidative Ring Cleavage in Jadomycin Biosynthesis: A Multistep Oxygenation Cascade in a Biosynthetic Black Box
Author(s) -
Rix Uwe,
Wang Chenchen,
Chen Yihua,
Lipata Fredilyn M.,
Remsing Rix Lily L.,
Greenwell Lisa M.,
Vining Leo C.,
Yang Keqian,
Rohr Jürgen
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400395
Subject(s) - biosynthesis , chemistry , cleavage (geology) , stereochemistry , oxygenase , biochemistry , dna , oxidative phosphorylation , biology , gene , paleontology , fracture (geology)
Backbone‐breaking oxygenase clusters . Inactivation of the oxygenase‐encoding genes jadH and jadG and structure elucidation of novel accumulation products illustrate the essential interactions within the biosynthetic protein complex that govern the oxygenation cascade leading to the cleavage of the angucycline backbone during jadomycin biosynthesis by Streptomyces venezuelae. These results also allow the elucidation of the dehydration sequence (shown) that yields the aromatic ring A of the jadomycin scaffold.