Premium
A Homologue of the Mycobacterium tuberculosis PapA5 Protein, Rif‐Orf20, Is an Acetyltransferase Involved in the Biosynthesis of Antitubercular Drug Rifamycin B by Amycolatopsis mediterranei S699
Author(s) -
Xiong Yeping,
Wu Xiumei,
Mahmud Taifo
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400387
Subject(s) - rifamycin , acetyltransferase , biosynthesis , mycobacterium tuberculosis , acyltransferases , enzyme , biochemistry , chemistry , stereochemistry , tuberculosis , biology , microbiology and biotechnology , antibiotics , medicine , gene , pathology , acetylation
Toward antitubercular drugs . Rif‐Orf20, a homologue of M. tuberculosis PapA5 protein, is an acetyltransferase that is responsible for the conversion of DMDARSV to DMRSV in rifamycin B biosynthesis. This enzyme, together with PapA5, represents a new class of acyltransferases. The enzyme also utilizes propionyl‐CoA as substrate to give rise to a new analogue of rifamycin that appears, from spectroscopic results, to have the structure 1 .