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Solid‐Phase Synthesis of Double‐Headed Epoxysuccinyl Activity‐Based Probes for Selective Targeting of Papain Family Cysteine Proteases
Author(s) -
Verhelst Steven H. L.,
Bogyo Matthew
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400377
Subject(s) - proteases , papain , deubiquitinating enzyme , cathepsin , cysteine , cathepsin b , moiety , chemistry , biochemistry , solid phase synthesis , enzyme , stereochemistry , peptide , ubiquitin , gene
Checking out cathepsins . A solid‐phase protocol was devised in order to synthesize activity‐based probes ( 1 ) that contain peptides on both ends of an epoxysuccinyl moiety. The feasibility of this strategy was illustrated by the synthesis of selective probes for cathepsin B, one of the major lysosomal Papain family cysteine proteases.