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Direct Quantitation of Poly(ADP‐Ribose) Polymerase (PARP) Activity as a Means to Distinguish Necrotic and Apoptotic Death in Cell and Tissue Samples
Author(s) -
Putt Karson S.,
Beilman Gregory J.,
Hergenrother Paul J.
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400330
Subject(s) - poly adp ribose polymerase , apoptosis , programmed cell death , polymerase , necrosis , microbiology and biotechnology , biology , enzyme , chemistry , biochemistry , genetics
PARP‐angel of death. The PARP‐1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP‐1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.