Direct Quantitation of Poly(ADP‐Ribose) Polymerase (PARP) Activity as a Means to Distinguish Necrotic and Apoptotic Death in Cell and Tissue Samples | Zendy

Putt Karson S. | Zendy; Beilman Gregory J. | Zendy; Hergenrother Paul J. | Zendy

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Direct Quantitation of Poly(ADP‐Ribose) Polymerase (PARP) Activity as a Means to Distinguish Necrotic and Apoptotic Death in Cell and Tissue Samples

Author(s) -

Putt Karson S.,

Beilman Gregory J.,

Hergenrother Paul J.

Publication year - 2005

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200400330

Subject(s) - poly adp ribose polymerase , apoptosis , programmed cell death , polymerase , necrosis , microbiology and biotechnology , biology , enzyme , chemistry , biochemistry , genetics

PARP‐angel of death. The PARP‐1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP‐1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.

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