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Directed Evolution of an Amine Oxidase for the Preparative Deracemisation of Cyclic Secondary Amines
Author(s) -
Carr Reuben,
Alexeeva Marina,
Dawson Michael J.,
GotorFernández Vicente,
Humphrey Cara E.,
Turner Nicholas J.
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400329
Subject(s) - directed evolution , amine gas treating , enzyme kinetics , amine oxidase , enzyme , chemistry , substrate (aquarium) , substrate specificity , oxidase test , stereochemistry , combinatorial chemistry , organic chemistry , biochemistry , active site , biology , gene , ecology , mutant
Off the rac . Directed evolution of the amine oxidase from Aspergillus niger (MAO‐N), by using 1‐methyltetrahydroisoquinoline as the target substrate, led to the identification of a variant enzyme (Asn336Ser/Ile246Met) that possessed a greater k cat value (5.5‐fold) than the parent as well as high enantioselectivity (see scheme). This new variant was used for the deracemisation of cyclic secondary amines on a preparative scale.