Directed Evolution of an Amine Oxidase for the Preparative Deracemisation of Cyclic Secondary Amines | Zendy

Carr Reuben | Zendy; Alexeeva Marina | Zendy; Dawson Michael J. | Zendy; GotorFernández Vicente | Zendy; Humphrey Cara E. | Zendy; Turner Nicholas J. | Zendy

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Directed Evolution of an Amine Oxidase for the Preparative Deracemisation of Cyclic Secondary Amines

Author(s) -

Carr Reuben,

Alexeeva Marina,

Dawson Michael J.,

GotorFernández Vicente,

Humphrey Cara E.,

Turner Nicholas J.

Publication year - 2005

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.200400329

Subject(s) - directed evolution , amine gas treating , enzyme kinetics , amine oxidase , enzyme , chemistry , substrate (aquarium) , substrate specificity , oxidase test , stereochemistry , combinatorial chemistry , organic chemistry , biochemistry , active site , biology , gene , ecology , mutant

Off the rac . Directed evolution of the amine oxidase from Aspergillus niger (MAO‐N), by using 1‐methyltetrahydroisoquinoline as the target substrate, led to the identification of a variant enzyme (Asn336Ser/Ile246Met) that possessed a greater k cat value (5.5‐fold) than the parent as well as high enantioselectivity (see scheme). This new variant was used for the deracemisation of cyclic secondary amines on a preparative scale.

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