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A New Embedded Process for Compartmentalized Cell‐Free Protein Expression and On‐line Detection in Microfluidic Devices
Author(s) -
Dittrich Petra S.,
Jahnz Michael,
Schwille Petra
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400321
Subject(s) - microfluidics , nanotechnology , fluorescence , emulsion , protein expression , chemistry , fluorescent protein , green fluorescent protein , confocal , cell culture , drop (telecommunication) , biophysics , materials science , biochemistry , computer science , biology , gene , physics , quantum mechanics , telecommunications , genetics , optics
Just a drop . In the field of biological research and biotechnology, fast and efficient screening for particular properties of minute quantities down to single‐particle level has become increasingly important. This work describes the implementation of a lab‐on‐chip device to generate small reaction containers, that is, a homodisperse water‐in‐oil emulsion, and cell‐free expression of the green fluorescent protein inside the aqueous droplets. Detection of the autofluorescent protein was performed by confocal fluorescence spectroscopy.