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Site‐Specific in vivo Labeling of Proteins for NMR Studies
Author(s) -
Deiters Alexander,
Geierstanger Bernhard H.,
Schultz Peter G.
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400319
Subject(s) - in vivo , amino acid , chemistry , mutagenesis , biochemistry , isotopic labeling , nuclear magnetic resonance spectroscopy , stereochemistry , biology , mutation , organic chemistry , genetics , gene
Unnatural amino acid mutagenesis was used for the site‐specific incorporation of an isotopically labeled amino acid, shown here, into a protein in vivo, and protein amounts sufficient for NMR studies were produced.
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