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Elucidation of the Glycosylation Sequence of Mithramycin Biosynthesis: Isolation of 3A‐Deolivosylpremithramycin B and Its Conversion to Premithramycin B by Glycosyltransferase MtmGII
Author(s) -
NureAlam Mohammad,
Méndez Carmen,
Salas José A.,
Rohr Jürgen
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400309
Subject(s) - glycosyltransferase , glycosylation , biosynthesis , mutant , biochemistry , isolation (microbiology) , chemistry , sequence (biology) , biology , enzyme , gene , bioinformatics
The last step at last . The isolation and structure elucidation of the novel premithramycin‐type compound 3A‐deolivosylpremithramycin B ( 1 ) allowed cross‐feeding experiments with this compound to two glycosyltransferase‐minus mutants of the mithramycin producer S. argillaceus ; this has solved the sequence of the last glycosylation steps of mithramycin biosynthesis.