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New Methods for the Generation of Carbohydrate Arrays on Glass Slides and Their Evaluation
Author(s) -
Biskup Moritz B.,
Müller Jan U.,
Weingart Ralf,
Schmidt Richard R.
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400300
Subject(s) - reductive amination , chemistry , carbohydrate , sugar , aldehyde , amination , glycoside , combinatorial chemistry , lectin , protecting group , carbohydrate chemistry , organic chemistry , glycopeptide , biochemistry , catalysis , alkyl , antibiotics
Glycosides, having spacers functionalized with an aldehyde or a carboxylic group, were immobilized through reductive amination or amidation, respectively, onto amino‐functionalized glass slides. Hybridization experiments with lectins exhibited very little nonspecific protein binding, hence precluding the necessity for the blocking of unreacted functional groups on the glass slide. The covalency and the concentration dependency of the sugar ligation to the glass slide were demonstrated; the reversibility and the selectivity of lectin–carbohydrate interactions were shown.