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Direct Observation of Anion‐Mediated Translocation of Fluorescent Oligoarginine Carriers into and across Bulk Liquid and Anionic Bilayer Membranes
Author(s) -
Sakai Naomi,
Takeuchi Toshihide,
Futaki Shiroh,
Matile Stefan
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400256
Subject(s) - bilayer , membrane , lipid bilayer , chemistry , biological membrane , biophysics , chloroform , lipid bilayer phase behavior , fluorescence , phase (matter) , organic chemistry , biochemistry , biology , physics , quantum mechanics
The recent hypothesis that counteranion‐mediated dynamic inversion of charge and solubility might contribute to diverse functions of oligoarginines in biomembranes was tested with two fluorescently labelled oligomers, FL‐R 8 , one of the most active cell‐penetrating peptides, and its longer version, FL‐R 16 . We report evidence for counteranion‐mediated phase transfer from water into bulk chloroform and anionic lipid‐bilayer membranes as well as reverse‐phase transfer from bulk chloroform and across intact lipid‐bilayer membranes into water. The differences found between FL‐R 8 and FL‐R 16 with regard to location in the bilayer and reverse‐phase transfer from bulk and lipid‐bilayer membranes into water implied that the reported results may be relevant for biological function.