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Extended Target Sequence Specificity of PNA–Minor‐Groove Binder Conjugates
Author(s) -
Nielsen Peter E.,
Frederiksen Karin,
Behrens Carsten
Publication year - 2005
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.200400251
Subject(s) - conjugate , minor groove , moiety , sequence (biology) , dna , peptide nucleic acid , chemistry , peptide , stereochemistry , nucleic acid , groove (engineering) , combinatorial chemistry , biochemistry , materials science , mathematical analysis , mathematics , metallurgy
Conjugal bliss . We show that a peptide nucleic acid–Hoechst conjugate (see scheme) kinetically and sequence preferentially guides the PNA moiety to target a binding site proximal to an A–T region that has an affinity for the minor‐groove binder. These results demonstrate a new strategy for constructing DNA recognition ligands composed of a sequence‐guiding domain that increases the target specificity and a DNA‐modification domain that determines the biological activity of the conjugate.